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Stimulation with female pheromone produced inward currents in patch-clamped neurons of male snake VNO slices in a dose-dependent manner. A typical response is illustrated in Figure 4. For each reaction, the amount of homogenate used was based on protein concentration only. None of the female VN organ sensory neurons responded to the pheromone, whereas three of 11 responded to ESS with induced inward current. The dose-response pheromone-induced inward currents were fit to the Hill equation, giving a Hill coefficient of 3.

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For each reaction, the amount of homogenate used was based on protein concentration only. The resulting methyl ketone residues were weighed on a digital scale Mettler AT and resuspended in 1 ml fresh hexane. Instead, she opted for a bottle of red wine. Although behavioral studies have established the critical involvement of the VN system in detection of this pheromone, the transduction mechanism by which the pheromone activates snake sensory neurons has yet to be elucidated. Grandma wants your fist and warm cum 12 min Older Woman Fun - 5. This pooled solution was subsequently used in the experiments as the stock pheromone solution. Action potential changes before, during, and after application of pheromone are illustrated in Figure 2.

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